Please use this identifier to cite or link to this item: http://lib.jncasr.ac.in:8080/jspui/handle/10572/509
Title: Isolation and Characterization of Binding Proteins for Retinol from the Cytosol, Nucleosol and Chromatin of the Oviduct Magnum of Laying Hens
Authors: Rao, M R S
Prasad, V R
Padmanaban, Govindarajan
Ganguly, Jagannath
Keywords: Animals
Cell Nucleus - analysis
Chickens
Chromatin - analysis
Chromatography
Gel
Cytosol - analysis
Estrogens - pharmacology
Oviducts - analysis
drug effects
Retinol-Binding Proteins - isolation & purification
Vitamin A - metabolism
Vitamin A Deficiency - metabolism
Issue Date: 1979
Publisher: The Biochemical Society
Citation: Biochemical Journal 183(3), 501–506 (1979)
Abstract: Protein fractions that bind retinol were isolated from the cytosol, nucleosol and chromatin of the oviduct magnum of laying hens. The proteins isolated from the three sources showed similar elution profiles on chromatography through Sephadex G-75 and G-50 columns, and comparable mobility during electrophoresis on sodium dodecyl sulphate/polyacrylamide gels. Their molecular weights were calculated to be around 14500. When oviducts from vitamin A-depleted and vitamin A-repleted immature chicks given oestrogen injections for 6 consecutive days were incubated with [3H]retinyl acetate, uptake of the radioactivity in the nuclei of the vitamin A-depleted tissue was severalfold higher than that in the nuclei from the vitamin A-repleted tissue.
Description: Restricted Access
URI: http://hdl.handle.net/10572/509
Other Identifiers: 0264-6021
Appears in Collections:Research Papers (M.R.S. Rao)

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