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dc.contributor.authorHeyer, Wolf-Dietrich-
dc.contributor.authorRao, M R S-
dc.contributor.authorErdile, Lorne F-
dc.contributor.authorKelly, ThomasJ-
dc.contributor.authorKolodner, R D-
dc.date.accessioned2012-02-15T08:20:23Z-
dc.date.available2012-02-15T08:20:23Z-
dc.date.issued1990-07-
dc.identifier0261-4189en_US
dc.identifier.citationEMBO Journal 9(7), 2321-2329 (1990)en_US
dc.identifier.urihttp://hdl.handle.net/10572/425-
dc.descriptionRestricted Accessen_US
dc.description.abstractSingle-stranded DNA binding proteins (SSBs) are known to play a role in DNA replication and recombination in prokaryotes. An SSB was previously purified from the yeast Saccharomyces cerevisiae. This SSB stimulated the activity of a cognate strand exchange protein (SEP1) in vitro suggesting a role in recombination. We have cloned and functionally analyzed the gene encoding this protein. DNA sequencing of the cloned DNA revealed a 621 amino acid open reading frame with a coding potential for a Mr 70,269 polypeptide. Highly significant amino acid homology was detected between this S.cerevisiae gene and the Mr 70,000 subunit polypeptide of human RP-A, a cellular protein essential for SV40 DNA replication in vitro. Therefore, we named the S.cerevisiae gene RPA1. RPA1 encodes an essential function in this organism as shown by tetrad analysis of heterozygous insertion mutants and is continuously required for mitotic growth. Cells lacking RPA1 accumulate as multiply budded cells with a single nucleus suggesting a defect in DNA replication.en_US
dc.description.urihttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC551959/?tool=pubmeden_US
dc.description.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=Retrieve&list_uids=2192864&dopt=abstractplusen_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.rights© 1990 Oxford University Pressen_US
dc.subjectAmino Acid Sequenceen_US
dc.subjectBase Sequenceen_US
dc.subjectChromosomesen_US
dc.subjectFungalen_US
dc.subjectCloningen_US
dc.subjectMolecularen_US
dc.subjectCrossesen_US
dc.subjectGeneticen_US
dc.subjectDNA-Binding Proteins-geneticsen_US
dc.subjectFungal Proteins-geneticsen_US
dc.subjectGene Libraryen_US
dc.subjectGenesen_US
dc.subjectFungalen_US
dc.subjectGenotypeen_US
dc.subjectHumansen_US
dc.subjectMacromolecular Substancesen_US
dc.subjectMolecular Sequence Dataen_US
dc.subjectMolecular Weighten_US
dc.subjectPlasmidsen_US
dc.subjectReplication Protein Aen_US
dc.subjectRestriction Mappingen_US
dc.subjectRibosomal Proteins-geneticsen_US
dc.subjectSaccharomyces cerevisiae-geneticsen_US
dc.subjectSaccharomyces cerevisiae-metabolismen_US
dc.subjectSaccharomyces cerevisiae Proteinsen_US
dc.subjectSequence Homologyen_US
dc.subjectNucleic Aciden_US
dc.titleAn essential Saccharomyces cerevisiae single-stranded DNA binding protein is homologous to the large subunit of human RP-Aen_US
dc.typeArticleen_US
Appears in Collections:Research Papers (M.R.S. Rao)

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