Please use this identifier to cite or link to this item: http://lib.jncasr.ac.in:8080/jspui/handle/10572/425
Title: An essential Saccharomyces cerevisiae single-stranded DNA binding protein is homologous to the large subunit of human RP-A
Authors: Heyer, Wolf-Dietrich
Rao, M R S
Erdile, Lorne F
Kelly, ThomasJ
Kolodner, R D
Keywords: Amino Acid Sequence
Base Sequence
Chromosomes
Fungal
Cloning
Molecular
Crosses
Genetic
DNA-Binding Proteins-genetics
Fungal Proteins-genetics
Gene Library
Genes
Fungal
Genotype
Humans
Macromolecular Substances
Molecular Sequence Data
Molecular Weight
Plasmids
Replication Protein A
Restriction Mapping
Ribosomal Proteins-genetics
Saccharomyces cerevisiae-genetics
Saccharomyces cerevisiae-metabolism
Saccharomyces cerevisiae Proteins
Sequence Homology
Nucleic Acid
Issue Date: Jul-1990
Publisher: Oxford University Press
Citation: EMBO Journal 9(7), 2321-2329 (1990)
Abstract: Single-stranded DNA binding proteins (SSBs) are known to play a role in DNA replication and recombination in prokaryotes. An SSB was previously purified from the yeast Saccharomyces cerevisiae. This SSB stimulated the activity of a cognate strand exchange protein (SEP1) in vitro suggesting a role in recombination. We have cloned and functionally analyzed the gene encoding this protein. DNA sequencing of the cloned DNA revealed a 621 amino acid open reading frame with a coding potential for a Mr 70,269 polypeptide. Highly significant amino acid homology was detected between this S.cerevisiae gene and the Mr 70,000 subunit polypeptide of human RP-A, a cellular protein essential for SV40 DNA replication in vitro. Therefore, we named the S.cerevisiae gene RPA1. RPA1 encodes an essential function in this organism as shown by tetrad analysis of heterozygous insertion mutants and is continuously required for mitotic growth. Cells lacking RPA1 accumulate as multiply budded cells with a single nucleus suggesting a defect in DNA replication.
Description: Restricted Access
URI: http://hdl.handle.net/10572/425
Other Identifiers: 0261-4189
Appears in Collections:Research Papers (M.R.S. Rao)

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