Please use this identifier to cite or link to this item:
|Title:||Conditional silencing of topoisomerase I gene of Mycobacterium tuberculosis validates its essentiality for cell survival|
Godbole, Adwait Anand
Karthik, Pullela V. D. N. B.
|Publisher:||Oxford University Press|
|Citation:||Ahmed, W; Menon, S; Godbole, AA; Karthik, PVDNB; Nagaraja, V, Conditional silencing of topoisomerase I gene of Mycobacterium tuberculosis validates its essentiality for cell survival. Fems Microbiology Letters 2014, 353 (2) 116-123, http://dx.doi.org/10.1111/1574-6968.12412|
FEMS Microbiology Letters
|Abstract:||Topoisomerases are an important class of enzymes for regulating the DNA transaction processes. Mycobacterium tuberculosis (Mtb) is one of the most formidable pathogens also posing serious challenges for therapeutic interventions. The organism contains only one type IA topoisomerase (Rv3646c), offering an opportunity to test its potential as a candidate drug target. To validate the essentiality of M.tuberculosis topoisomerase I (TopoI(Mt)) for bacterial growth and survival, we have generated a conditionally regulated strain of topoI in Mtb. The conditional knockdown mutant exhibited delayed growth on agar plate. In liquid culture, the growth was drastically impaired when TopoI expression was suppressed. Additionally, novobiocin and isoniazid showed enhanced inhibitory potential against the conditional mutant. Analysis of the nucleoid revealed its altered architecture upon TopoI depletion. These studies establish the essentiality of TopoI for the M.tuberculosis growth and open up new avenues for targeting the enzyme.|
|Appears in Collections:||Research Articles (Nagaraja, V.)|
Files in This Item:
|556.94 kB||Adobe PDF||View/Open Request a copy|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.