Please use this identifier to cite or link to this item: http://lib.jncasr.ac.in:8080/jspui/handle/10572/2480
Title: Genetic Diversity in HIV-1 Subtype C LTR from Brazil and Mozambique Generates New Transcription Factor-Binding Sites
Authors: Boullosa, Jose
Bachu, Mahesh
Bila, Dulce
Ranga, Udaykumar
Sueffert, Theodoro
Sasazawa, Tomoko
Tanuri, Amilcar
Keywords: Virology
HIV-1
Subtype C
Ltr
Insertion
Nfkb
Rbeiii
Brazil
Rio Grande Do Sul
Parana
Mozambique
Mfnlp
Immunodeficiency-Virus Type-1
Replicative Fitness
Southern Brazil
Sequences
Identification
Predominance
Recombinant
Crf02-Ag
Promoter
Disease
Issue Date: 2014
Publisher: Mdpi Ag
Citation: Boullosa, J; Bachu, M; Bila, D; Ranga, U; Suffert, T; Sasazawa, T; Tanuri, A, Genetic Diversity in HIV-1 Subtype C LTR from Brazil and Mozambique Generates New Transcription Factor-Binding Sites. Viruses-Basel 2014, 6 (6) 2495-2504, http://dx.doi.org/10.3390/v6062495
Viruses-Basel
6
6
Abstract: The HIV-1 subtype C has been substituting the subtype B population in southern Brazil. This phenomenon has been previously described in other countries, suggesting that subtype C may possess greater fitness than other subtypes. The HIV-1 long-terminal repeat (LTR) is an important regulatory region critical for the viral life cycle. Sequence insertions immediately upstream of the viral enhancer are known as the most frequent naturally occurring length polimorphisms (MFNLP). Previous reports demonstrated that the MFNLP could lead to the duplication of transcription factor binding sites (TFBS) enhancing the activity of the HIV-1 subtype C LTR. Here, we amplified and sequenced the LTR obtained from proviral DNA samples collected from patients infected with subtype C from the Southern Region of Brazil (naive or treatment failure) and Mozambique (only naive). We confirm the presence of different types of insertions in the LTR sequences of both the countries leading to the creation of additional TFBS. In the Brazilian clinical samples, the frequency of the sequence insertion was significantly higher in subjects experiencing treatment failure than in antiretroviral naive patients.
Description: Open Access
URI: http://hdl.handle.net/10572/2480
ISSN: 1999-4915
Appears in Collections:Research Papers (Ravi Manjithaya)

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