Please use this identifier to cite or link to this item: http://lib.jncasr.ac.in:8080/jspui/handle/10572/2477
Title: ARF1-GTP regulates Asrij to provide endocytic control of Drosophila blood cell homeostasis
Authors: Khadilkar, Rohan J.
Rodrigues, Diana
Mote, Ridim Dadasaheb
Sinha, Arghyashree Roychowdhury
Kulkarni, Vani
Magadi, Srivathsa Subramanya
Inamdar, Maneesha S.
Keywords: Hematopoietic Progenitor Maintenance
Adp-Ribosylation Factor
Lymph Gland
Melanogaster
Protein
Activation
Networks
Steppke
Signals
Model
Issue Date: 2014
Publisher: National Academy of Sciences
Citation: Khadilkar, RJ; Rodrigues, D; Mote, RD; Sinha, AR; Kulkarni, V; Magadi, SS; Inamdar, MS, ARF1-GTP regulates Asrij to provide endocytic control of Drosophila blood cell homeostasis. Proceedings of The National Academy of Sciences of The United States of America 2014, 111 (13) 4898-4903, http://dx.doi.org/10.1073/pnas.1303559111
Proceedings of The National Academy of Sciences of The United States of America
111
13
Abstract: Drosophila melanogaster larval hematopoiesis is a well-established model to study mechanisms that regulate hematopoietic niche maintenance and control of blood cell precursor (prohemocyte) differentiation. Molecules that perturb niche function affect the balance between prohemocytes and differentiated hemocytes. The conserved hemocyte-specific endosomal protein Asrij is essential for niche function and prohemocyte maintenance. Elucidating how subcellular trafficking molecules can regulate signaling presents an important challenge. Here we show that Asrij function is mediated by the Ras family GTPase Arf79F, the Drosophila homolog of ADP ribosylation factor 1 (ARF1), essential for clathrin coat assembly, Golgi architecture, and vesicular trafficking. ARF1 is expressed in the larval lymph gland and in circulating hemocytes and interacts with Asrij. ARF1-depleted lymph glands show loss of niche cells and prohemocyte maintenance with increased differentiation. Inhibiting ARF1 activation by knocking down its guanine nucleotide exchange factor (Gartenzwerg) or overexpressing its GTPAse-activating protein showed that ARF1-GTP is essential for regulating niche size and maintaining stemness. Activated ARF1 regulates Asrij levels in blood cells thereby mediating Asrij function. Asrij controls crystal cell differentiation by affecting Notch trafficking. ARF1 perturbation also leads to aberrant Notch trafficking and the Notch intracellular domain is stalled in sorting endosomes. Thus, ARF1 can regulate Drosophila blood cell homeostasis by regulating Asrij endocytic function. ARF1 also regulates signals arising from the niche and differentiated cells by integrating the insulin-mediated and PDGF-VEGF receptor signaling pathways. We propose that the conserved ARF1-Asrij endocytic axis modulates signals that govern hematopoietic development. Thus, Asrij affords tissue-specific control of global mechanisms involved in molecular traffic.
Description: Restricted Access
URI: http://hdl.handle.net/10572/2477
ISSN: 0027-8424
Appears in Collections:Research Papers (Maneesha S. Inamdar)

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