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dc.contributor.authorModak, Rahul
dc.contributor.authorDas Mitra, Susweta
dc.contributor.authorVasudevan, Madavan
dc.contributor.authorKrishnamoorthy, Paramanandhan
dc.contributor.authorKumar, Manoj
dc.contributor.authorBhat, Akshay V.
dc.contributor.authorBhuvana, Mani
dc.contributor.authorGhosh, Sankar K.
dc.contributor.authorShome, Bibek R.
dc.contributor.authorKundu, Tapas Kumar
dc.date.accessioned2017-02-17T05:09:15Z-
dc.date.available2017-02-17T05:09:15Z-
dc.date.issued2014
dc.identifier.citationModak, R; Das Mitra, S; Vasudevan, M; Krishnamoorthy, P; Kumar, M; Bhat, AV; Bhuvana, M; Ghosh, SK; Shome, BR; Kundu, TK, Epigenetic response in mice mastitis: Role of histone H3 acetylation and microRNA(s) in the regulation of host inflammatory gene expression during Staphylococcus aureus infection. Clinical Epigenetics 2014, 6, 12 http://dx.doi.org/10.1186/1868-7083-6-12en_US
dc.identifier.citationClinical Epigeneticsen_US
dc.identifier.citation6en_US
dc.identifier.issn1868-7083
dc.identifier.urihttp://hdl.handle.net/10572/2330-
dc.descriptionOpen Accessen_US
dc.description.abstractBackground: There is renewed interest towards understanding the host-pathogen interaction in the light of epigenetic modifications. Although epithelial tissue is the major site for host-pathogen interactions, there is handful of studies to show how epithelial cells respond to pathogens. Bacterial infection in the mammary gland parenchyma induces local and subsequently systemic inflammation that results in a complex disease called mastitis. Globally Staphylococcus aureus is the single largest mastitis pathogen and the infection can ultimately result in either subclinical or chronic and sometimes lifelong infection. Results: In the present report we have addressed the differential inflammatory response in mice mammary tissue during intramammary infection and the altered epigenetic context induced by two closely related strains of S. aureus, isolated from field samples. Immunohistochemical and immunoblotting analysis showed strain specific hyperacetylation at histone H3K9 and H3K14 residues. Global gene expression analysis in S. aureus infected mice mammary tissue revealed a selective set of upregulated genes that significantly correlated with the promoter specific, histone H3K14 acetylation. Furthermore, we have identified several differentially expressed known miRNAs and 3 novel miRNAs in S. aureus infected mice mammary tissue by small RNA sequencing. By employing these gene expression data, an attempt has been made to delineate the gene regulatory networks in the strain specific inflammatory response. Apparently, one of the isolates of S. aureus activated the NF-kB signaling leading to drastic inflammatory response and induction of immune surveillance, which could possibly lead to rapid clearance of the pathogen. The other strain repressed most of the inflammatory response, which might help in its sustenance in the host tissue. Conclusion: Taken together, our studies shed substantial lights to understand the mechanisms of strain specific differential inflammatory response to S. aureus infection during mastitis. In a broader perspective this study also paves the way to understand how certain bacteria can evade the immune surveillance and cause sustained infection while others are rapidly cleared from the host body.en_US
dc.description.urihttp://dx.doi.org/10.1186/1868-7083-6-12en_US
dc.language.isoEnglishen_US
dc.publisherBiomed Central Ltden_US
dc.rights@Biomed Central Ltd, 2014en_US
dc.subjectOncologyen_US
dc.subjectEpigenetic Modificationsen_US
dc.subjectHistoneen_US
dc.subjectAcetylationen_US
dc.subjectMastitisen_US
dc.subjectS. Aureus Infectionen_US
dc.subjectMicro-RNAen_US
dc.subjectGene Expressionen_US
dc.subjectInflammatory Responseen_US
dc.subjectChromatin Immunoprecipitationen_US
dc.subjectMammary Epithelial-Cellsen_US
dc.subjectEscherichia-Colien_US
dc.subjectBlood-Cellsen_US
dc.subjectMouseen_US
dc.subjectGlanden_US
dc.subjectRelevanceen_US
dc.subjectPathogensen_US
dc.subjectCytokineen_US
dc.subjectPromoteren_US
dc.titleEpigenetic response in mice mastitis: Role of histone H3 acetylation and microRNA(s) in the regulation of host inflammatory gene expression during Staphylococcus aureus infectionen_US
dc.typeArticleen_US
Appears in Collections:Research Papers (Tapas K. Kundu)

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