Please use this identifier to cite or link to this item: http://lib.jncasr.ac.in:8080/jspui/handle/10572/2328
Title: Centromeric histone variant CENP-A represses acetylation-dependent chromatin transcription that is relieved by histone chaperone NPM1
Authors: Shandilya, Jayasha
Senapati, Parijat
Hans, Fabienne
Menoni, Herve
Bouvet, Philippe
Dimitrov, Stefan
Angelov, Dimitar
Kundu, Tapas Kumar
Keywords: Biochemistry & Molecular Biology
Acetylation
Cenp-A
Histone Chaperone
Npm1
Transcription
In-Vitro
Nucleosome Reconstitution
Protein-A
Expression
Purification
Complex
HJURP
Issue Date: 2014
Publisher: Oxford University Press
Citation: Shandilya, J; Senapati, P; Hans, F; Menoni, H; Bouvet, P; Dimitrov, S; Angelov, D; Kundu, TK, Centromeric histone variant CENP-A represses acetylation-dependent chromatin transcription that is relieved by histone chaperone NPM1. Journal of Biochemistry 2014, 156 (4) 221-227, http://dx.doi.org/10.1093/jb/mvu034
Journal of Biochemistry
156
4
Abstract: Mammalian centromeric histone H3 variant, CENP-A, is involved in maintaining the functional integrity and epigenetic inheritance of the centromere. CENP-A causes transcriptional repression of centromeric chromatin through an unknown mechanism. Here, we report that reconstituted CENP-A nucleosomes are amenable to ATP-dependent SWI/SNF-mediated remodelling but are less permissive to acetylation and acetylation-dependent in vitro chromatin transcription. Remarkably, the transcriptional repression of the CENP-A chromatinized template could be relieved by the ectopic addition of histone chaperone, nucleophosmin.
Description: Restricted Access
URI: http://hdl.handle.net/10572/2328
ISSN: 0021-924X
Appears in Collections:Research Papers (Tapas K. Kundu)

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