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dc.contributor.authorHoque, Jiaul
dc.contributor.authorAdhikary, Utsarga
dc.contributor.authorYadav, Vikas
dc.contributor.authorSamaddar, Sandip
dc.contributor.authorKonai, Mohini Mohan
dc.contributor.authorPrakash, Relekar Gnaneshwar
dc.contributor.authorPararnanandham, Krishnamoorthy
dc.contributor.authorShome, Bibek R.
dc.contributor.authorSanyal, Kaustuv
dc.contributor.authorHaldar, Jayanta
dc.identifier.citationHoque, J.; Adhikary, U.; Yadav, V.; Samaddar, S.; Konai, M. M.; Prakash, R. G.; Pararnanandham, K.; Shome, B. R.; Sanyal, K.; Haldar, J., Chitosan Derivatives Active against Multidrug-Resistant Bacteria and Pathogenic Fungi: In Vivo Evaluation as Topical Antimicrobials. Molecular Pharmaceutics 2016, 13 (10), 3578-3589
dc.identifier.citationMolecular Pharmaceuticsen_US
dc.descriptionRestricted Accessen_US
dc.description.abstractThe continuous rise of antimicrobial resistance and the dearth of new antibiotics in the clinical pipeline raise an urgent call for the development of potent antimicrobial agents. Cationic chitosan derivatives, N-(2-hydroxypropyl)-3-trimethylammonium chitosan chlorides (HTCC), have been widely studied as potent antibacterial agents. However, their systemic structure-activity relationship, activity toward drug-resistant bacteria and fungi, and mode of action are very rare. Moreover, toxicity and efficacy of these polymers under in vivo conditions are yet to be established. Herein, we investigated antibacterial and antifungal efficacies of the HTCC polymers against multidrug resistant bacteria including clinical isolates and pathogenic fungi, studied their mechanism of action, and evaluated cytotoxic and antimicrobial activities in vitro and in vivo. The polymers were found to be active against both bacteria and fungi (MIC = 125-250 mu g/mL) and displayed rapid microbicidal kinetics, killing pathogens within 60-120 min. Moreover, the polymers were shown to target both bacterial and fungal cell membrane leading to membrane disruption and found to be effective in hindering bacterial resistance development. Importantly, very low toxicity toward human erythrocytes (HC50 = >10000 mu g/mL) and embryo kidney cells were observed for the cationic polymers in vitro. Further, no inflammation toward skin tissue was observed in vivo for the most active polymer even at 200 mg/kg when applied on the mice skin. In a murine model of superficial skin infection, the polymer showed significant reduction of methicillin-resistant Staphylococcus aureus (MRSA) burden (3.2 log MRSA reduction at 100 mg/kg) with no to minimal inflammation. Taken together, these selectively active polymers show promise to be used as potent antimicrobial agents in topical and other infections.en_US
dc.publisherAmerican Chemical Societyen_US
dc.rights@American Chemical Society, 2016en_US
dc.subjectResearch & Experimental Medicineen_US
dc.subjectPharmacology & Pharmacyen_US
dc.subjectantimicrobial polymeren_US
dc.subjectcationic chitosan derivativesen_US
dc.subjectdrug-sensitive and drug-resistant bacteriaen_US
dc.subjectpathogenic fungien_US
dc.subjecttopical infectionen_US
dc.subjectantimicrobial resistanceen_US
dc.subjectAntifungal Drug-Resistanceen_US
dc.subjectQuaternary N-Alkylen_US
dc.subjectAntibacterial Activityen_US
dc.subjectEradicate Biofilmsen_US
dc.subjectNylon-3 Polymersen_US
dc.titleChitosan Derivatives Active against Multidrug-Resistant Bacteria and Pathogenic Fungi: In Vivo Evaluation as Topical Antimicrobialsen_US
Appears in Collections:Research Papers (Jayanta Haldar)
Research Papers (Kaustuv Sanyal)

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