Please use this identifier to cite or link to this item: http://lib.jncasr.ac.in:8080/jspui/handle/10572/2298
Title: Chitosan Derivatives Active against Multidrug-Resistant Bacteria and Pathogenic Fungi: In Vivo Evaluation as Topical Antimicrobials
Authors: Hoque, Jiaul
Adhikary, Utsarga
Yadav, Vikas
Samaddar, Sandip
Konai, Mohini Mohan
Prakash, Relekar Gnaneshwar
Pararnanandham, Krishnamoorthy
Shome, Bibek R.
Sanyal, Kaustuv
Haldar, Jayanta
Keywords: Research & Experimental Medicine
Pharmacology & Pharmacy
antimicrobial polymer
cationic chitosan derivatives
drug-sensitive and drug-resistant bacteria
pathogenic fungi
topical infection
antimicrobial resistance
Antifungal Drug-Resistance
Quaternary N-Alkyl
Antibacterial Activity
Molecular-Mechanisms
Eradicate Biofilms
Candida-Albicans
Nylon-3 Polymers
Peptides
Chloride
Agents
Issue Date: 2016
Publisher: American Chemical Society
Citation: Hoque, J.; Adhikary, U.; Yadav, V.; Samaddar, S.; Konai, M. M.; Prakash, R. G.; Pararnanandham, K.; Shome, B. R.; Sanyal, K.; Haldar, J., Chitosan Derivatives Active against Multidrug-Resistant Bacteria and Pathogenic Fungi: In Vivo Evaluation as Topical Antimicrobials. Molecular Pharmaceutics 2016, 13 (10), 3578-3589 http://dx.doi.org/10.1021/acs.molpharmaceut.6b00764
Molecular Pharmaceutics
13
10
Abstract: The continuous rise of antimicrobial resistance and the dearth of new antibiotics in the clinical pipeline raise an urgent call for the development of potent antimicrobial agents. Cationic chitosan derivatives, N-(2-hydroxypropyl)-3-trimethylammonium chitosan chlorides (HTCC), have been widely studied as potent antibacterial agents. However, their systemic structure-activity relationship, activity toward drug-resistant bacteria and fungi, and mode of action are very rare. Moreover, toxicity and efficacy of these polymers under in vivo conditions are yet to be established. Herein, we investigated antibacterial and antifungal efficacies of the HTCC polymers against multidrug resistant bacteria including clinical isolates and pathogenic fungi, studied their mechanism of action, and evaluated cytotoxic and antimicrobial activities in vitro and in vivo. The polymers were found to be active against both bacteria and fungi (MIC = 125-250 mu g/mL) and displayed rapid microbicidal kinetics, killing pathogens within 60-120 min. Moreover, the polymers were shown to target both bacterial and fungal cell membrane leading to membrane disruption and found to be effective in hindering bacterial resistance development. Importantly, very low toxicity toward human erythrocytes (HC50 = >10000 mu g/mL) and embryo kidney cells were observed for the cationic polymers in vitro. Further, no inflammation toward skin tissue was observed in vivo for the most active polymer even at 200 mg/kg when applied on the mice skin. In a murine model of superficial skin infection, the polymer showed significant reduction of methicillin-resistant Staphylococcus aureus (MRSA) burden (3.2 log MRSA reduction at 100 mg/kg) with no to minimal inflammation. Taken together, these selectively active polymers show promise to be used as potent antimicrobial agents in topical and other infections.
Description: Restricted Access
URI: http://hdl.handle.net/10572/2298
ISSN: 1543-8384
Appears in Collections:Research Papers (Jayanta Haldar)
Research Papers (Kaustuv Sanyal)

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