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Title: Stimuli-responsive colorimetric and NIR fluorescence combination probe for selective reporting of cellular hydrogen peroxide
Authors: Narayanaswamy, Nagarjun
Narra, Sivakrishna
Nair, Raji R.
Saini, Deepak Kumar
Kondaiah, Paturu
Govindaraju, T.
Keywords: Chemistry
Thiols Reducing Repair
H2O2 Oxidative Stress
Living Cells
Issue Date: 2016
Publisher: Royal Society of Chemistry
Citation: Narayanaswamy, N.; Narra, S.; Nair, R. R.; Saini, D. K.; Kondaiah, P.; Govindaraju, T., Stimuli-responsive colorimetric and NIR fluorescence combination probe for selective reporting of cellular hydrogen peroxide. Chemical Science 2016, 7 (4), 2832-2841
Chemical Science
Abstract: Hydrogen peroxide (H2O2) is a key reactive oxygen species and a messenger in cellular signal transduction apart from playing a vital role in many biological processes in living organisms. In this article, we present phenyl boronic acid-functionalized quinone-cyanine (QCy-BA) in combination with AT-rich DNA (exogenous or endogenous cellular DNA), i.e., QCy-BA subset of DNA as a stimuli-responsive NIR fluorescence probe for measuring in vitro levels of H2O2. In response to cellular H2O2 stimulus, QCy-BA converts into QCy-DT, a one-donor-two-acceptor (D2A) system that exhibits switch-on NIR fluorescence upon binding to the DNA minor groove. Fluorescence studies on the combination probe QCy-BA subset of DNA showed strong NIR fluorescence selectively in the presence of H2O2. Furthermore, glucose oxidase (GOx) assay confirmed the high efficiency of the combination probe QCy-BA subset of DNA for probing H2O2 generated in situ through GOx-mediated glucose oxidation. Quantitative analysis through fluorescence plate reader, flow cytometry and live imaging approaches showed that QCy-BA is a promising probe to detect the normal as well as elevated levels of H2O2 produced by EGF/Nox pathways and post-genotoxic stress in both primary and senescent cells. Overall, QCy-BA, in combination with exogenous or cellular DNA, is a versatile probe to quantify and image H2O2 in normal and disease-associated cells.
Description: Open Access
ISSN: 2041-6520
Appears in Collections:Research Papers (Govindaraju, T.)

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