Please use this identifier to cite or link to this item: http://lib.jncasr.ac.in:8080/jspui/handle/10572/2197
Title: Surface-Functionalized Silk Fibroin Films as a Platform To Guide Neuron-like Differentiation of Human Mesenchymal Stem Cells
Authors: Manchineella, Shivaprasad
Thrivikraman, Greeshma
Basu, Bikramjit
Govindaraju, T.
Keywords: Materials Science
silk fibroin
laminin peptide
surface functionalization
biomaterials
tissue engineering
transdifferentiation
neuron-like stem cells
Extracellular-Matrix
In-Vitro
Regenerative Medicine
Growth-Factors
Retinoic-Acid
Tissue
Biomaterials
Fate
Nanofibers
Substrate
Issue Date: 2016
Publisher: American Chemical Society
Citation: Manchineella, S.; Thrivikraman, G.; Basu, B.; Govindaraju, T., Surface-Functionalized Silk Fibroin Films as a Platform To Guide Neuron-like Differentiation of Human Mesenchymal Stem Cells. Acs Applied Materials & Interfaces 2016, 8 (35), 22849-22859 http://dx.doi.org/10.1021/acsami.6b06403
ACS Applied Materials & Interfaces
8
35
Abstract: Surface interactions at the biomaterial cellular interface determine the proliferation and differentiation of stem cells. Manipulating such interactions through the surface chemistry of scaffolds renders control over directed stem cell differentiation into the cell lineage of interest. This approach is of central importance for stem cell-based tissue engineering and regenerative therapy applications. In the present study, silk fibroin films (SFFs) decorated with integrin-binding laminin peptide motifs (YIGSR and GYIGSR) were prepared and employed for in vitro adult stem cell-based neural tissue engineering applications. Functionalization of SFFs with short peptides showcased the peptide sequence and nature of functionalization-dependent differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs). Intriguingly, covalently functionalized SFFs with GYIGSR hexapeptide (CL2-SFF) supported hMSC proliferation and maintenance in an undifferentiated pluripotent state and directed the differentiation of hMSCs into neuron-like cells in the presence of a biochemical cue, on-demand. The observed morphological changes were further corroborated by the up-regulation of neuronal-specific marker gene expression (MAP2, TUBB3, NEFL), confirmed through semiquantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The enhanced proliferation and on-demand directed differentiation of adult stem cells (hMSCs) by the use of an economically viable short recognition peptide (GYIGSR), as opposed to the integrin recognition protein laminin, establishes the potential of SFFs for neural tissue engineering and regenerative therapy applications.
Description: Restricted Access
URI: http://hdl.handle.net/10572/2197
ISSN: 1944-8244
Appears in Collections:Research Papers (Govindaraju, T.)

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