Please use this identifier to cite or link to this item: http://lib.jncasr.ac.in:8080/jspui/handle/10572/2192
Title: Apicoplast fatty acid synthesis is essential for pellicle formation at the end of cytokinesis in Toxoplasma gondii
Authors: Martins-Duarte, Erica S.
Carias, Maira
Vommaro, Rossiane
Surolia, Namita
de Souza, Wanderley
Keywords: Cell Biology
Cell division
Bradyzoite
Triclosan
Apicomplexa
Cleavage furrow
Carrier Protein Reductase
De-Novo Synthesis
Plasmodium-Falciparum
Apicomplexan Parasites
Long-Chain
Freeze Fracture
Drug Target
Lipoic Acid
Host-Cell
Biosynthesis
Issue Date: 2016
Publisher: Company Of Biologists Ltd
Citation: Martins-Duarte, E. S.; Carias, M.; Vommaro, R.; Surolia, N.; de Souza, W., Apicoplast fatty acid synthesis is essential for pellicle formation at the end of cytokinesis in Toxoplasma gondii. Journal of Cell Science 2016, 129 (17), 3320-3331 http://dx.doi.org/10.1242/jcs.185223
Journal of Cell Science
129
17
Abstract: The apicomplexan protozoan Toxoplasma gondii, the causative agent of toxoplasmosis, harbors an apicoplast, a plastid-like organelle with essential metabolic functions. Although the FASII fatty acid biosynthesis pathway located in the apicoplast is essential for parasite survival, the cellular effects of FASII disruption in T. gondii had not been examined in detail. Here, we combined light and electron microscopy techniques-including focused ion beam scanning electron microscopy (FIB-SEM)-to characterize the effect of FASII disruption in T. gondii, by treatment with the FASII inhibitor triclosan or by inducible knockdown of the FASII component acyl carrier protein. Morphological analyses showed that FASII disruption prevented cytokinesis completion in T. gondii tachyzoites, leading to the formation of large masses of 'tethered' daughter cells. FIB-SEM showed that tethered daughters had a mature basal complex, but a defect in new membrane addition between daughters resulted in incomplete pellicle formation. Addition of exogenous fatty acids to medium suppressed the formation of tethered daughter cells and supports the notion that FASII is essential to generate lipid substrates required for the final step of parasite division.
Description: Restricted Access
URI: http://hdl.handle.net/10572/2192
ISSN: 0021-9533
Appears in Collections:Research Papers (Namita Surolia)

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