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|Title:||Generation of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR|
Inamdar, Maneesha S.
Biotechnology & Applied Microbiology
|Publisher:||Elsevier Science Bv|
|Citation:||Shetty, R.; Inamdar, M. S., Generation of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR. Stem Cell Research 2016, 16 (2), 271-273 http://dx.doi.org/10.1016/j.scr.2015.12.043|
Stem Cell Research
|Abstract:||Human embryonic stem cell line BJNhem20-TetR was generated using non-viral method. The construct pCAG-TetRnls was transfected using microporation procedure. BJNhem20-TetR can subsequently be transfected with any vector harbouring a TetO (Tet operator) sequence to generate doxycycline based inducible line. For example, in human embryonic stem cells, the pSuperior based TetO system has been transfected into a TetR containing line to generate OCT4 knockdown cell line (Zafarana et al., 2009). Thus BJNhem20-TetR can be used as a tool to perturb gene expression in human embryonic stem cells. (C) 2016 The Authors. Published by Elsevier B.V.|
|Appears in Collections:||Research Papers (Maneesha S. Inamdar)|
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