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Title: Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On
Authors: Shetty, Deeti K.
Shetty, Ronak
Inamdar, Maneesha S.
Keywords: Cell Biology
Biotechnology & Applied Microbiology
Issue Date: 2016
Publisher: Elsevier Science Bv
Citation: Shetty, D. K.; Shetty, R.; Inamdar, M. S., Generation of OCIAD1 inducible overexpression human embryonic stem cell line: BJNhem20-OCIAD1-Tet-On. Stem Cell Research 2016, 16 (2), 246-248
Stem Cell Research
Abstract: Human embryonic stem cell line BJNhem20-OCIAD1-Tet-On was generated using non-viral method. The constructs pCAG-Tet-On and pTRE-Tight vector driving OCIAD1 expression were transfected using microporation procedure. pCAG-Tet-On cells can be used for inducible expression of any coding sequence cloned into pTRE-Tight vector. For example, in human embryonic stem cells, Tet-On system has been used to generate SOX2 overexpression cell line (Adachi et al., 2010). (C) 2016 The Authors. Published by Elsevier B.V.
Description: Restricted Access
ISSN: 1873-5061
Appears in Collections:Research Papers (Maneesha S. Inamdar)

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