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dc.contributor.authorShetty, Deeti K.
dc.contributor.authorInamdar, Maneesha S.
dc.date.accessioned2017-01-24T06:32:23Z-
dc.date.available2017-01-24T06:32:23Z-
dc.date.issued2016
dc.identifier.citationShetty, D. K.; Inamdar, M. S., Generation of a transgenic human embryonic stem cell line ectopically expressing the endosomal protein Asrij that regulates pluripotency in mouse embryonic stem cells: BJNhem20-Asrij. Stem Cell Research 2016, 16 (2), 331-333 http://dx.doi.org/10.1016/j.scr.2015.12.044en_US
dc.identifier.citationStem Cell Researchen_US
dc.identifier.citation16en_US
dc.identifier.citation2en_US
dc.identifier.issn1873-5061
dc.identifier.urihttp://hdl.handle.net/10572/2187-
dc.descriptionRestricted Accessen_US
dc.description.abstractAsrij is an endocytic protein expressed in mouse embryonic stem cells (mESCs) and is essential for maintenance of stemness of mESCs (Mukhopadhyay et al., 2003; Sinha et al., 2013). Its human ortholog named Ovarian Carcinoma Immuno-reactive Antigen domain containing protein 1 (OCIAD1) is 85% identical. We ectopically expressed Asrij in epiblast stage equivalent-human embryonic stem cells (hESCs) to test for induction of naive pluripotency in primed pluripotent cells. The construct pCAG-Asrij was introduced into hESCs by microporation. Ectopic expression of Asrij in BJNhem20 hESC line was performed by selecting for plasmid transfection, followed by stable cell line generation. (C) 2016 The Authors. Published by Elsevier B.V.en_US
dc.description.uri1876-7753en_US
dc.description.urihttp://dx.doi.org/10.1016/j.scr.2015.12.044en_US
dc.language.isoEnglishen_US
dc.publisherElsevier Science Bven_US
dc.rights@Elsevier Science Bv, 2016en_US
dc.subjectCell Biologyen_US
dc.subjectBiotechnology & Applied Microbiologyen_US
dc.titleGeneration of a transgenic human embryonic stem cell line ectopically expressing the endosomal protein Asrij that regulates pluripotency in mouse embryonic stem cells: BJNhem20-Asrijen_US
dc.typeArticleen_US
Appears in Collections:Research Papers (Maneesha S. Inamdar)

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