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Title: Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato
Authors: Shetty, Deeti K.
Inamdar, Maneesha S.
Keywords: Cell Biology
Biotechnology & Applied Microbiology
Issue Date: 2016
Publisher: Elsevier Science Bv
Citation: Shetty, D. K.; Inamdar, M. S., Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato. Stem Cell Research 2016, 16 (2), 290-292
Stem Cell Research
Abstract: Human embryonic stem cell line BJNhem20-pCAG-tdTomato was generated using non-viral method. The construct pCAG-tdTomato was transfected using microporation procedure. This fluorescent hESC line can help to study heterogeneity within individual cells in hESC colonies by enabling live tracking of their growth, migration and differentiation properties. This cell line also serves as a resource for additional transgene introduction/knock-out/knock-in generation in a fluorescent background and allows ease of analysis in studies involving cell mixing. (C) 2016 The Authors. Published by Elsevier B.V.
Description: Restricted Access
ISSN: 1873-5061
Appears in Collections:Research Papers (Maneesha S. Inamdar)

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