Please use this identifier to cite or link to this item: http://lib.jncasr.ac.in:8080/jspui/handle/10572/2182
Title: Repeat-Associated Fission Yeast-Like Regional Centromeres in the Ascomycetous Budding Yeast Candida tropicalis
Authors: Chatterjee, Gautam
Sankaranarayanan, Sundar Ram
Guin, Krishnendu
Thattikota, Yogitha
Padmanabhan, Sreedevi
Siddharthan, Rahul
Sanyal, Kaustuv
Keywords: Genetics & Heredity
Cenp-A Chromatin
Schizosaccharomyces-Pombe
Saccharomyces-Cerevisiae
Dna-Sequences
Caenorhabditis-Elegans
Kinetochore Proteins
Chromosome Evolution
Functional-Analysis
Neurospora-Crassa
Rapid Evolution
Issue Date: 2016
Publisher: Public Library of Science
Citation: Chatterjee, G.; Sankaranarayanan, S. R.; Guin, K.; Thattikota, Y.; Padmanabhan, S.; Siddharthan, R.; Sanyal, K., Repeat-Associated Fission Yeast-Like Regional Centromeres in the Ascomycetous Budding Yeast Candida tropicalis. Plos Genetics 2016, 12 (2), 28 http://dx.doi.org/10.1371/journal.pgen.1005839
PLoS Genetics
12
2
Abstract: The centromere, on which kinetochore proteins assemble, ensures precise chromosome segregation. Centromeres are largely specified by the histone H3 variant CENP-A (also known as Cse4 in yeasts). Structurally, centromere DNA sequences are highly diverse in nature. However, the evolutionary consequence of these structural diversities on de novo CENP-A chromatin formation remains elusive. Here, we report the identification of centromeres, as the binding sites of four evolutionarily conserved kinetochore proteins, in the human pathogenic budding yeast Candida tropicalis. Each of the seven centromeres comprises a 2 to 5 kb non-repetitive mid core flanked by 2 to 5 kb inverted repeats. The repeat-associated centromeres of C. tropicalis all share a high degree of sequence conservation with each other and are strikingly diverged from the unique and mostly non-repetitive centromeres of related Candida species-Candida albicans, Candida dubliniensis, and Candida lusitaniae. Using a plasmid-based assay, we further demonstrate that pericentric inverted repeats and the underlying DNA sequence provide a structural determinant in CENP-A recruitment in C. tropicalis, as opposed to epigenetically regulated CENP-A loading at centromeres in C. albicans. Thus, the centromere structure and its influence on de novo CENP-A recruitment has been significantly rewired in closely related Candida species. Strikingly, the centromere structural properties along with role of pericentric repeats in de novo CENP-A loading in C. tropicalis are more reminiscent to those of the distantly related fission yeast Schizosaccharomyces pombe. Taken together, we demonstrate, for the first time, fission yeast-like repeat-associated centromeres in an ascomycetous budding yeast.
Description: Open Access
URI: http://hdl.handle.net/10572/2182
ISSN: 1553-7404
Appears in Collections:Research Papers (Kaustuv Sanyal)

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