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Title: Multifunctional human transcriptional coactivator protein PC4 is a substrate of Aurora kinases and activates the Aurora enzymes
Authors: Dhanasekaran, Karthigeyan
Kumari, Sujata
Boopathi, Ramachandran
Shima, Hiroki
Swaminathan, Amrutha
Bachu, Mahesh
Ranga, Udaykumar
Igarashi, Kazuhiko
Kundu, Tapas Kumar
Keywords: Biochemistry & Molecular Biology
Aurora kinase
cell cycle
positive coactivator 4
spindle organization
Chromatin-Associated Protein
Structural Basis
Issue Date: 2016
Publisher: Wiley-Blackwell
Citation: Dhanasekaran, K.; Kumari, S.; Boopathi, R.; Shima, H.; Swaminathan, A.; Bachu, M.; Ranga, U.; Igarashi, K.; Kundu, T. K., Multifunctional human transcriptional coactivator protein PC4 is a substrate of Aurora kinases and activates the Aurora enzymes. Febs Journal 2016, 283 (6), 968-985
FEBS Journal
Abstract: Positive coactivator 4 (PC4), a human transcriptional coactivator, is involved in diverse processes like chromatin organization and transcription regulation. It is hyperphosphorylated during mitosis, with unknown significance. For the first time, we demonstrate the function of PC4 outside the nucleus upon nuclear envelope breakdown. A fraction of PC4 associates with Aurora A and Aurora B and undergoes phosphorylation, following which PC4 activates both Aurora A and B to sustain optimal kinase activity to maintain the phosphorylation gradient for the proper functioning of the mitotic machinery. This mitotic role is evident in PC4 knockdown cells where the defects are rescued only by the catalytically active Aurora kinases, but not the kinase-dead mutants. Similarly, the PC4 phosphodeficient mutant failed to rescue such defects. Hence, our observations establish a novel mitotic function of PC4 that might be dependent on Aurora kinase-mediated phosphorylation.
Description: Restricted Access
ISSN: 1742-464X
Appears in Collections:Research Papers (Tapas K. Kundu)
Research Papers (Udaykumar Ranga)

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