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Title: FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC
Authors: Richard, John Lalith Charles
Shukla, Manu Shubhdarshan
Menoni, Herve
Ouararhni, Khalid
Lone, Imtiaz Nisar
Roulland, Yohan
Papin, Christophe
Ben Simon, Elsa
Kundu, Tapas Kumar
Hamiche, Ali
Angelov, Dimitar
Dimitrov, Stefan
Keywords: Genetics & Heredity
Nucleosome Core Particle
Histone Octamer
H2A-H2B Displacement
Chaperone Fact
Issue Date: 2016
Publisher: Public Library of Science
Citation: Richard, J. L. C.; Shukla, M. S.; Menoni, H.; Ouararhni, K.; Lone, I. N.; Roulland, Y.; Papin, C.; Ben Simon, E.; Kundu, T.; Hamiche, A.; Angelov, D.; Dimitrov, S., FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC. Plos Genetics 2016, 12 (7), 21
PLoS Genetics
Abstract: FACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair. Here, we present evidence that FACT could be directly involved in Base Excision Repair and elucidate the chromatin remodeling mechanisms of FACT during BER. We found that, upon oxidative stress, FACT is released from transcription related protein complexes to get associated with repair proteins and chromatin remodelers from the SWI/SNF family. We also showed the rapid recruitment of FACT to the site of damage, coincident with the glycosylase OGG1, upon the local generation of oxidized DNA. Interestingly, FACT facilitates uracil-DNA glycosylase in the removal of uracil from nucleosomal DNA thanks to an enhancement in the remodeling activity of RSC. This discloses a novel property of FACT wherein it has a co-remodeling activity and strongly enhances the remodeling capacity of the chromatin remodelers. Altogether, our data suggest that FACT may acts in concert with RSC to facilitate excision of DNA lesions during the initial step of BER.
Description: Open Access
ISSN: 1553-7404
Appears in Collections:Research Papers (Tapas K. Kundu)

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