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|Title:||14-3-3 gamma Prevents Centrosome Amplification and Neoplastic Progression|
Hosing, Amol S.
Kundu, Tapas Kumar
Dalal, Sorab N.
|Publisher:||Nature Publishing Group|
|Citation:||Mukhopadhyay, A.; Sehgal, L.; Bose, A.; Gulvady, A.; Senapati, P.; Thorat, R.; Basu, S.; Bhatt, K.; Hosing, A. S.; Balyan, R.; Borde, L.; Kundu, T. K.; Dalal, S. N., 14-3-3 gamma Prevents Centrosome Amplification and Neoplastic Progression. Scientific Reports 2016, 6, 19 http://dx.doi.org/10.1038/srep26580|
|Abstract:||More than 80% of malignant tumors show centrosome amplification and clustering. Centrosome amplification results from aberrations in the centrosome duplication cycle, which is strictly coordinated with DNA-replication-cycle. However, the relationship between cell-cycle regulators and centrosome duplicating factors is not well understood. This report demonstrates that 14-3-3 gamma localizes to the centrosome and 14-3-3 gamma loss leads to centrosome amplification. Loss of 14-3-3 gamma results in the phosphorylation of NPM1 at Thr-199, causing early centriole disjunction and centrosome hyperduplication. The centrosome amplification led to aneuploidy and increased tumor formation in mice. Importantly, an increase in passage of the 14-3-3 gamma-knockdown cells led to an increase in the number of cells containing clustered centrosomes leading to the generation of pseudo-bipolar spindles. The increase in pseudo-bipolar spindles was reversed and an increase in the number of multi-polar spindles was observed upon expression of a constitutively active 14-3-3-binding-defective-mutant of cdc25C (S216A) in the 14-3-3 gamma knockdown cells. The increase in multi-polar spindle formation was associated with decreased cell viability and a decrease in tumor growth. Our findings uncover the molecular basis of regulation of centrosome duplication by 14-3-3 gamma and inhibition of tumor growth by premature activation of the mitotic program and the disruption of centrosome clustering.|
|Appears in Collections:||Research Papers (Tapas K. Kundu)|
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