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Title: An impediment to random walk: trehalose microenvironment drives preferential endocytic uptake of plasmonic nanoparticles
Authors: Siddhanta, Soumik
Zheng, Chao
Narayana, Chandrabhas
Barman, Ishan
Keywords: Chemistry
Surface-Enhanced Raman
Gold Nanoparticles
Silver Nanoparticles
Protein Corona
Issue Date: 2016
Publisher: Royal Society of Chemistry
Citation: Siddhanta, S.; Zheng, C.; Narayana, C.; Barman, I., An impediment to random walk: trehalose microenvironment drives preferential endocytic uptake of plasmonic nanoparticles. Chemical Science 2016, 7 (6), 3730-3736
Chemical Science
Abstract: Developing effective theranostic nanoplex platforms for personalized disease treatment necessitates an understanding of and the ability to control live cell-nanoparticle interactions. However, aggregation of nanoparticles on the cell surface and their subsequent internalization is sparsely understood and adversely impact cellular recognition and viability. Here we report a facile method of precisely modulating the aggregation and uptake for silver nanoparticles without altering their surface geometry or functionalization. Exploiting the stabilization properties of trehalose, our approach enables uptake of nanoparticles while reducing aggregation on cell surface and maintaining cell viability. Electron microscopy reveals the larger utilization of endosomal structures in the trehalose-rich environment compared to the nanoparticles' "free" cytosolic diffusion patterns in the control group. Additionally, in the presence of trehalose, plasmon-enhanced Raman spectroscopy confirms the preservation of the protein structure in the vicinity of the nanoparticles reinforcing the promise of the proposed route for label-free, multiplexed intracellular monitoring.
Description: Open Access
ISSN: 2041-6520
Appears in Collections:Research Articles (Chandrabhas N.)

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