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Title: Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction
Authors: Meetei, Amom Ruhikanta
Rao, M R S
Keywords: Multiple Site-Specific Mutations
Polymerase Chain Reaction
Issue Date: 6-Nov-2001
Publisher: Humana Press Inc.
Citation: Methods in Molecular Biology, vol. 182: In Vitro Mutagenesis Protocols, 2nd ed. Edited by: J. Braman, Humana Press Inc., Totowa, NJ, 95-102 (2001)
Abstract: Generating mutant proteins has become a routine strategy for molecular and structural biologists, to understand the structure-function relationship of a given protein of interest. In this, site-directed mutagenesis has proven to be a valuable technique. After the advent of polymerase chain reaction (PCR), PCR- mediated mutagenesis has been the method of choice to change from one amino acid to another, which has replaced the classic Kunkel’s method (1).
Description: Restricted access.
Other Identifiers: 1064-3745
Appears in Collections:Research Papers (M.R.S. Rao)

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